T7 DNA Ligase from New England Biolabs

Product Specs
ItemT7 DNA Ligase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0318S
Quantity100000 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Protocol for the saturation and multiplexing of genetic variants using CRISPR-Cas9STAR ProtocolsDecember 1, 2023Sounak Sahu, Teresa Sullivan, Eileen Southon, Dylan Caylor, Josephine Geh, Shyam K. Sharan(high fidelity) enzyme,New England Biolabs,Cat# R0539S T7 DNA ligase enzyme,New England Biolabs,Cat# M0318S ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, PX458-Cas9-2A-GFP,Addgene # 62988 (kind gift

(2) SLC3A2 N-glycosylation and Golgi remodeling regulate SLC7A amino acid exchangers and stress mitigationJournal of Biological ChemistryDecember 1, 2023Cunjie Zhang, Massiullah Shafaq-Zadah, Judy Pawling, Geoffrey G. Hesketh, Estelle Dransart, Karina Pacholczyk, Joseph Longo, Anne-Claude Gingras, Linda Z. Penn, Ludger Johannes, et al.(F5277-76). CRISPR/CAS9 Reagents: FastDigest BbsI (#FD1014) from Thermo Scientific; T7 DNA ligase (#M0318S), T4 PNK (#M0201S), ATP (#P0756S) from NEB; Plasmid-Safe ATP-Dependent DNase (#E3101K) from Epicentre;

(3) PRR14 organizes H3K9me3-modified heterochromatin at the nuclear laminaNucleus (Austin, Tex.)December 1, 2023Kiseleva AA, Cheng YC, Smith CL, Katz RA, Poleshko A.were sub-cloned into the expression vector pCMV6-AN-mGFP using T7 ligase (New England Biolabs, cat# M0318S) according to the manufacturer’s instructions. To make Doxycycline-inducible vectors, PRR14-GFP full-length

(4) Systematic investigation of allelic regulatory activity of schizophrenia-associated common variantsCell GenomicsOctober 11, 2023Jessica C. McAfee, Sool Lee, Jiseok Lee, Jessica L. Bell, Oleh Krupa, Jessica Davis, Kimberly Insigne, Marielle L. Bond, Nanxiang Zhao, Alan P. Boyle, et al.that contains the sequences for MluI, SpeI, and EcoRI restriction sites using T7 DNA ligase (NEB, cat#M0318S). The ligated plasmid was transformed into Endura electrocompetent cells (Lucigen, cat#60242-1) via

(5) Protocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cellsSTAR ProtocolsJune 1, 2023Nannan Kong, Ying Wai Chan,New England Biolabs,Cat# M0492L FastDigest Bpil,Thermo Fisher,Cat# FD1014 T7 DNA ligase,New England Biolabs,Cat# M0318S BamHI-HF,New England Biolabs,Cat# 3136S ScaI-HF,New England Biolabs,Cat# R3122S Quick CIP,New England Biolabs

(6) Rapid, scalable, combinatorial genome engineering by marker-less enrichment and recombination of genetically engineered loci in yeastCell Reports MethodsMay 22, 2023Mudabir Abdullah, Brittany M. Greco, Jon M. Laurent, Riddhiman K. Garge, Daniel R. Boutz, Michelle Vandeloo, Edward M. Marcotte, Aashiq H. Kachroo,Thermo Fisher Scientific,Cat#FD0454 ATP solution (10mM),Thermo Fisher Scientific,Cat#AM8110G T7 ligase,NEB,Cat#M0318S T4 ligase,NEB,Cat#M0202S R-Zymolase,Zymoresearch,Cat#E1006 G418,Thermo Fisher Scientific,Cat#LS10131027 "Zymolase,

(7) A TurboID-based proximity labelling approach for identifying the DNA-binding proteinsSTAR ProtocolsMarch 1, 2023Xia-fei Wei, Shan Li, Jie-li Hu,Thermo Scientific,Cat#BY5 BsmbI-V2,New England Biolabs,Cat#R0739S T7 DNA ligase,New England Biolabs,Cat#M0318S PrimeSTAR Max DNA Polymerase,TAKARA,Cat#R045B "Collagen I, rat tail",BD BIOCOAT,Cat#354236 Biotin,Sigma-Aldrich,Cat#B4501 Protease

(8) In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1PLOS ONEFebruary 16, 2023Marc Blanch-Asensio, Sourik Dey, Shrikrishnan SankaranQuick Blunting Kit (NEB GmbH, Germany, Art. No. E1201S) and the T4 DNA Ligase enzyme (NEB GmbH, Germany, Art. No. M0318S) were used. L. plantarum WCFS1 electrocompetent cell preparation Wild-type L. plantarum WCFS1 was

(9) Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cellsNature CommunicationsDecember 24, 2022Qin Peng, Ziliang Huang, Kun Sun, Yahan Liu, Chi Woo Yoon, Reed E. S. Harrison, Danielle L. Schmitt, Linshan Zhu, Yiqian Wu, Ipek Tasan, et al.ATP (P0756S, New England BioLabs), 0.5 μl BbsI (R3539S, New England BioLabs), 0.3 μl T7 DNA ligase (M0318S, New England BioLabs), 1 μl 200 nM Annealed oligos, add ddH2O up to 10 μl. Incubate at 37 °C for 15

(10) Protocol for gene characterization in Aspergillus niger using 5S rRNA-CRISPR-Cas9-mediated Tet-on inducible promoter exchangeSTAR ProtocolsDecember 16, 2022Xiaomei Zheng, Timothy Cairns, Ping Zheng, Vera Meyer, Jibin SunBIOTECH,Cat#DP305 T4 DNA Ligase Reaction Buffer,New England Biolabs,Cat#B0202S T4 DNA Ligase,New England Biolabs,Cat#M0318S BbsI,New England Biolabs,Cat#R0535S FastPfu Fly PCR SuperMix,TransGen Biotech,Cat#AS231-01 ,, Experimental models

(11) Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factorsPlant MethodsDecember 1, 2022Shaunak Kar, Yogendra Bordiya, Nestor Rodriguez, Junghyun Kim, Elizabeth C. Gardner, Jimmy D. Gollihar, Sibum Sung, Andrew D. Ellingtonenzymes were used for the assemblies – BsaI-v2 (NEB #R3733S), Esp3I (NEB #R0734S) and T7 DNA ligase (NEB #M0318S). Plant material, bacterial infiltration Nicotiana benthamiana and Arabidopsis thaliana plants were

(12) TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagyNature CommunicationsSeptember 26, 2022Chengyang Li, Patrick Duckney, Tong Zhang, Yanshu Fu, Xin Li, Johan Kroon, Geert De Jaeger, Yunjiang Cheng, Patrick J. Hussey, Pengwei WangPstI/BamHI-digested pTRB1 and pTRB2 fragments were inserted respectively using T7 DNA Ligase (NEB, M0318S). For generation of recombinant TRB1-6xHIS protein, full length TRB1 CDS was PCR amplified from the

(13) Antibody escape and cryptic cross-domain stabilization in the SARS-CoV-2 Omicron spike proteinCell Host & MicrobeSeptember 14, 2022Kamyab Javanmardi et al.handler. To each well, we added the following Golden Gate reaction mixture: 0.25 μL of T7 DNA Ligase (NEB M0318S), 0.25 μL of AarI (Thermo Fisher ER1582), 0.2 μL AarI Oligo (Thermo Fisher), 1 μL T4 DNA Ligase Buffer

(14) A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeastSTAR ProtocolsJune 17, 2022Daniele Novarina, Andriana Koutsoumpa, Andreas Milias-Argeitiskit,MACHEREY-NAGEL,Cat#740609 T4 DNA Ligase Reaction Buffer,New England Biolabs,Cat#B0202S T7 DNA Ligase,New England Biolabs,Cat#M0318S BsmBI,New England Biolabs,Cat#R0580S BsaI,New England Biolabs,Cat#R0535S EcoRI-HF,New England Biolabs,Cat#R3101S Q5 High-Fidelity DNA Polymerase

(15) Identification of STAU1 as a regulator of HBV replication by TurboID-based proximity labelingiScienceJune 17, 2022Wei XF, Fan SY, Wang YW, Li S, Long SY, Gan CY, Li J, Sun YX, Guo L, Wang PY, et al.,Thermo Scientific,Cat#BY5 BsmbI-V2,New England Biolabs,Cat#R0739S T7 DNA ligase,New England Biolabs,Cat#M0318S T5 exonuclease,New England Biolabs,Cat#M0363 PrimeSTAR Max DNA Polymerase,TAKARA,Cat#R045B IPTG,Beyotime,Cat#ST098 Pronase,Roche,Cat#10165921001 Micrococcal nuclease

(16) Detection of CCR5Δ32 Mutant Alleles in Heterogeneous Cell Mixtures Using Droplet Digital PCRFrontiers in Molecular BiosciencesFebruary 21, 2022Alyona Sorokina, Alexander Artyuhov, Alexandra Goltsova, Erdem Dashinimaevannealed oligonucleotides into the backbone using T7 DNA ligase (NEB, Ipswich, United States; Cat. no. M0318S) in a C1000 Touch Thermal Cycler (Bio-Rad, Hercules, United States) according to the protocol: for 10

(17) SPARCLE, a p53-induced lncRNA, controls apoptosis after genotoxic stress by promoting PARP-1 cleavageMolecular cellFebruary 17, 2022Meza-Sosa KF, Miao R, Navarro F, Zhang Z, Zhang Y, Hu JJ, Hartford CCR, Li XL, Pedraza-Alva G, Pérez-Martínez L, et al.S11494 T4 RNA ligase 1 (ssRNA ligase),New England Biolabs,Cat# M0204S T7 ligase,New England Biolabs,Cat# M0318S TaqMan® Universal PCR Master Mix no AmpErase® UNG,ThermoFisher Scientific,Cat# 4364341 Trichloroacetic

(18) Protocols for marker-free gene knock-out and knock-down in Kluyveromyces marxianus using CRISPR/Cas9FEMS Yeast ResearchJanuary 29, 2022Arun S Rajkumar, John P Morrissey(B0202S, New England Biolabs)T4 polynucleotide kinase (M0201S/L, New England Biolabs).T7 DNA ligase (M0318S/L, New England Biolabs)BsaI-HFv2 (R3733S/L, New England Biolabs).Cytiva Illustra ExoProStar 1-Step (Cytiva

(19) Rapid characterization of spike variants via mammalian cell surface displayMolecular CellDecember 16, 2021Kamyab Javanmardi et al.destination plate received the following Golden Gate reaction mixture: 0.25 μL of T7 DNA Ligase (NEB M0318S), 0.25 μL of AarI (Thermo Fisher ER1582), 0.2 μL AarI Oligo (Thermo Fisher), 1 μL T4 DNA Ligase Buffer

(20) Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systemsNature CommunicationsDecember 1, 2021Timothy C. Yu et al.1.5 h at 37 °C. Insert (library) and vector (pLibAcceptorV2) were ligated using T7 DNA Ligase (NEB #M0318S), incubating at room temperature for 1 h. The plasmid was then transformed into DH5α electrocompetent

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T7 DNA Ligase

T7 DNA Ligase from New England Biolabs

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T7 DNA Ligase from New England Biolabs

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